The emergence of antimicrobial resistance (AMR) has led to a pressing need for alternative antimicrobial agents that are both potent and less vulnerable to the development of resistance. Cationic antimicrobial biomacromolecules (CAMBs) area promising family of antimicrobial agents that can be designed to exhibit low toxicity while remaining effective across a broad spectrum of bacteria. Using an electrostatic layer-by-layer (LbL) process, the activity of antimicrobial agents, including CAMBs, on the surfaces of biomedical implants or wound dressings is preserved and allows for sustained release over several days.Additionally, nanoparticles containing current or new antimicrobials with LbL coatings can be designed to accumulate in inflamed regions of the lung to penetrate established biofilms, reduce toxicity, and improve treatment outcomes.