Technology Platforms
Microscopy and Imaging
Immunohistochemistry and fluorescence-based microscopy
Instrumentation
- Zeiss LSM700 Confocal Microscope
- Leica Instruments Microtome
- 4 laser system (405nm, 488nm, 555nm, 639nm)
- z-stack and time-lapse capability
Support
- Paraffin- and cryo-sectioning
- Immunohistochemistry (H&E staining)
- Fluorescence panel design and methods development
- Image acquisition
Scope
- Relative (qPCR) and Absolute (ddPCR) quantitation of gene expression
- Pathogen detection
- Viral load quantitation
- Expression knockdown quantitation (siRNA / RNAi, MicroRNA)
- Rare allele detection (ddPCR)